Effect of Calcium Cation on Thermophilic Acylamino Acid-releasing Enzyme Ape1547 from Aeropyrum pernix K1 |
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| Authors: | ZHOU Jun XIE Gui-qiu CAO Shu-gui GAI Ren-jun |
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| Institution: | [1]Key Laboratory for Molecular Enzymology and Engineering, Ministry of Education [2]School of Pharmaceutical Sciences, Jilin University, Changchun 130021, P. R. China |
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| Abstract: | The gene of enzyme(Ape1547) was cloned from hyperthermophilic archaeon Aeropyrum pernix K1 and expressed in Escherichia coil.The effect of calcium cation on the properties of Ape1547 was studied.Ape1547 exhibits both peptidase activity and esterase activity.The fluorescence spectrum shows that calcium cation quenches the fluorescence of the enzyme through static quenching mechanism,indicating that calcium cation was bound to the enzyme.Based on the study of calcium cation on CD ellipticity of Ape1547 by cir... |
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| Keywords: | Acylamino acid-releasing enzyme Aeropyrum pernix K1 Conformational change Calcium cation Artiele ID 1005-9040(2009)-04-513-05 |
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