Fluorescence enhancement effect for the determination of adenosine 5'-monophosphate with 9-anthracene carboxylic acid-cetyl trimethyl ammonium bromide system |
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Authors: | Khot Mahadev S Desai Netaji K Kolekar Govind B Patil Shivajirao R |
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Institution: | (1) Ch. Sambhaji Raje Sainik School, Jamge (Khed), Ratnagiri, 415709, Maharashtra, India;(2) Fluorescence Spectroscopy Laboratory, Department of Chemistry, Shivaji University, Kolhapur, 416 004, Maharashtra, India |
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Abstract: | A fluorimetric method based on fluorescence enhancement effect was developed for the determination of adenosine 5′-monophosphate
(AMP) with 9-anthracene carboxylic acid (9-ANCA)–cetyl trimethyl ammonium bromide (CTAB) system. Fluorescence intensity of
9-ANCA was decreased by the addition of CTAB but addition of AMP again rose the intensity of 9-ANCA gradually. The observed
fluorescence enhancement is attributed to the competitive binding reaction of 9-ANCA and adenosine to CTAB. The enhancement
in the fluorescence intensity was found proportional to the concentration of AMP over the range 2.0 × 10−4 to 1.2 × 10−3 mol dm−3. The ion pair complex is formed spontaneously between 9-ANCA and CTAB. Since the binding interaction is larger for the adenosine–CTAB
pair, the fluorophore 9-ANCA will be released. The quantum yield of free 9-ANCA is higher therefore its fluorescence observed
at 417 nm wavelength is enhanced. This mechanism of competitive molecular interaction is further confirmed by conductometric
measurements. The method was applied successfully for the determination of AMP from pharmaceutical sample. The method is more
selective, sensitive and relatively free from interferences. |
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