Lanthanide-based protease activity sensors for time-resolved fluorescence measurements |
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Authors: | Mizukami Shin Tonai Kazuhiro Kaneko Masahiro Kikuchi Kazuya |
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Institution: | Division of Advanced Science and Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan. |
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Abstract: | Lanthanide-based luminescent sensors are noteworthy because their long-lifetime luminescence enables time-resolved fluorescence measurements. After exploring suitable antenna groups, we designed and synthesized lanthanide-based luminogenic sensors detecting protease activities. This sensor yielded strong luminescence on addition of proteases such as calpain I and leucine aminopeptidase (LAP). Since the luminescence lifetimes of the probes were very long, the sensors could be applied to time-resolved measurements that exclude background fluorescence signals derived from proteins or other impurities. This sensing principle could be applicable to general time-resolved fluorescence assays for other proteases. |
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