Design, synthesis, and evaluation of in vivo potency and selectivity of epoxysuccinyl-based inhibitors of papain-family cysteine proteases |
| |
Authors: | Sadaghiani Amir Masoud Verhelst Steven H L Gocheva Vasilena Hill Kimberly Majerova Eva Stinson Sherman Joyce Johanna A Bogyo Matthew |
| |
Institution: | Department of Pathology, Stanford School of Medicine, Stanford, CA 94305, USA. |
| |
Abstract: | The papain-family cathepsins are cysteine proteases that are emerging as promising therapeutic targets for a number of human disease conditions ranging from osteoporosis to cancer. Relatively few selective inhibitors for this family exist, and the in vivo selectivity of most existing compounds is unclear. We present here the synthesis of focused libraries of epoxysuccinyl-based inhibitors and their screening in crude tissue extracts. We identified a number of potent inhibitors that display selectivity for endogenous cathepsin targets both in vitro and in vivo. Importantly, the selectivity patterns observed in crude extracts were generally retained in vivo, as assessed by active-site labeling of tissues from treated animals. Overall, this study identifies several important compound classes and highlights the use of activity-based probes to assess pharmacodynamic properties of small-molecule inhibitors in vivo. |
| |
Keywords: | |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|