Dodecyl maltoside detergent improves resolution of hepatic membrane proteins in two-dimensional gels. |
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Authors: | F Witzmann B Jarnot D Parker |
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Institution: | Molecular Anatomy Laboratory, Indiana University-Purdue University, Indianapolis, Columbus 47203. |
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Abstract: | This report describes the incorporation of an alkyl maltoside detergent in two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) sample lysis buffer in order to improve resolution of protein patterns separated by nonequilibrium pH gradient electrophoresis. Membrane-associated proteins with alkaline isoelectric points form horizontal streaks on two-dimensional electrophoretograms when solubilized with conventional nonionic detergent. Dodecyl maltoside enhances protein delipidation during solubilization and improves pattern resolution and protein mobility. |
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