Spectroscopic Investigation on the Synergistic Effects of Ultrasound and Dioxopromethazine Hydrochloride on Protein |
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| Authors: | Ling-Ling He Xin Wang Bin Liu Jun Wang Ya-Guang Sun Shu-Kun Xu |
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| Institution: | (1) Department of Chemistry, Northeastern University, Shenyang, 110819, People’s Republic of China;(2) College of Applied Chemistry, Shenyang University of Chemical Technology, Shenyang, 110142, People’s Republic of China;(3) School of Pharmaceutical Sciences, Liaoning University, Shenyang, 110036, People’s Republic of China;(4) Department of Chemistry, Liaoning University, Shenyang, 110036, People’s Republic of China; |
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| Abstract: | The bovine serum albumin (BSA) was selected as a target molecule, the sonodynamic damage to protein in the presence of dioxopromethazine
hydrochloride (DPZ) and its mechanism were studied by means of absorption and fluorescence spectra. The results of hyperchromic
effect of absorption spectra and quenching of intrinsic fluorescence spectra indicated that the synergistic effects of ultrasound
and DPZ could induce the damage of BSA molecules. The damage degree of BSA molecules increased with the increase of ultrasonic
irradiation time and DPZ concentration. The results of synchronous fluorescence and three-dimensional fluorescence spectra
further confirmed that the synergistic effects of ultrasound and DPZ induced the damage of BSA molecules. The results of oxidation-extraction
photometry with several reactive oxygen species (ROS) scavengers indicated that the damage of BSA molecules could be mainly
due to the generation of ROS, in which both 1O2 and ·OH were the important mediators of the ultrasound-inducing BSA molecules damage in the presence of DPZ. |
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