| 基于毛细管液滴技术的血液乙型肝炎病毒DNA提取 |
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| 引用本文： | 梁广铁,王秋平,王维,刘大渔,周小棉.基于毛细管液滴技术的血液乙型肝炎病毒DNA提取[J].分析化学,2011,39(5):670-674. |
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| 作者姓名： | 梁广铁 王秋平 王维 刘大渔 周小棉 |
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| 作者单位： | 广州医学院附属广州市第一人民医院检验科临床化学技术研究室,广州,510180 |
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| 基金项目： | "十一五"重大专项，国家自然科学基金，广州市医药卫生科技重点项目 |
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| 摘 要： | 基于毛细管液滴技术,建立了提取血液中乙型肝炎病毒(HBV)DNA的方法.方法的基本原理是向聚四氟乙烯毛细管中连续引人油相和水相溶液时,由于表面张力作用,可以形成稳定的油包水型液滴.依次引人含有不同试样的液滴,在毛细管中完成进样、DNA结合、洗涤以及洗脱等过程.实验表明,采用蛋白酶K裂解和提高洗脱温度有利于提高DNA回收...
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| 关 键 词： | 微全核酸分析系统 DNA提取 液滴 毛细管 乙型肝炎病毒 |
Droplet-based Blood Hepatitis B Virus DNA Extraction in a Capillary |
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| MANG Guang-Tie,WANG Qiu-Ping,WANG Wei,LIU Da-Yu,ZHOU Xiao-Mian.Droplet-based Blood Hepatitis B Virus DNA Extraction in a Capillary[J].Chinese Journal of Analytical Chemistry,2011,39(5):670-674. |
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| Authors: | MANG Guang-Tie WANG Qiu-Ping WANG Wei LIU Da-Yu ZHOU Xiao-Mian |
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| Institution: | LIANG Guang-Tie,WANG Qiu-Ping,WANG Wei,LIU Da-Yu,ZHOU Xiao-Mian(Laboratory of Clinical Chemical Technology,Guangzhou First Municipal People's Hospital,Affiliated Hospital of Guangzhou Medical College,Guangzhou 510180) |
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| Abstract: | A novel in-capillary droplet-based DNA extraction method was developed,using the principle of water-in-oil droplet formation in the highly hydrophobic polytetrafluoroethylene capillary.By sequentially introducing droplets containing sample or reagents,all the steps related to blood hepatitis B virus(HBV) DNA extraction,including sample injection,DNA binding,magnetic beads rinsing and DNA elution were continuously implemented in the capillary.Experimental results showed that proteinase K lysis and elevated e... |
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| Keywords: | Micro-total nucleic acid analysis system Deoxyribonucleic acid extraction Droplet Capillary Hepatitis B virus |
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