| 基于发光金纳米粒子荧光增强法测定溶菌酶 |
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| 引用本文： | 钱章生,刘玫瑰,田大慧,郝丹,朱昌青.基于发光金纳米粒子荧光增强法测定溶菌酶[J].分析化学,2011,39(5):611-616. |
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| 作者姓名： | 钱章生 刘玫瑰 田大慧 郝丹 朱昌青 |
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| 作者单位： | 安徽师范大学化学与材料科学学院,安徽省化学与生物传感重点实验室,芜湖,241000 |
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| 摘 要： | 参照文献方法合成了BSA修饰的水溶性发光金纳米粒子,并考察了其与溶菌酶之间的相互作用.依据溶菌酶对金纳米粒子的发光增强现象,建立了测定溶菌酶的荧光新方法.考察了发光金纳米粒子的浓度、pH值、反应时间及共存物质对测定的影响.优化条件为:发光金纳米粒子浓度4.0×10(-6)mol/L,pH 7.0、反应时间10 min....
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| 关 键 词： | 溶菌酶 发光金纳米粒子 荧光方法 |
Fluorescence Enhancement Method for Determination of Lysozyme Using Fluorescent Gold Nanoparticles as Probe |
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| QIAN Zhang-Sheng,LIU Mei-Gui,TIAN Da-Hui,HAO Dan,ZHU Chang-Qing.Fluorescence Enhancement Method for Determination of Lysozyme Using Fluorescent Gold Nanoparticles as Probe[J].Chinese Journal of Analytical Chemistry,2011,39(5):611-616. |
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| Authors: | QIAN Zhang-Sheng LIU Mei-Gui TIAN Da-Hui HAO Dan ZHU Chang-Qing |
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| Institution: | QIAN Zhang-Sheng,LIU Mei-Gui,TIAN Da-Hui,HAO Dan,ZHU Chang-Qing(Anhui Key Laboratory of Chemo-Biosensing,College of Chemical and Materials Science,Anhui Normal University,Wuhu 241000) |
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| Abstract: | As lysozyme has the property of dissolving some bacteria,it can be applied in medical treatment,biological engineering,and especially in food antisepsis to replace chemically synthesized ones.Therefore,the development of a simple analytical method for lysozyme assay is very important.Here,water-soluble BSA-modified fluorescent gold nanoparticles(GNPs) were prepared according to the literature,and their interaction with lysozyme was investigated.A novel fluorescence method for the determination of lysozyme h... |
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| Keywords: | Lysozyme Fluorescent gold nanoparticles Fluorescent method |
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