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Isoelectric focusing on cellulose acetate membranes: an up-date on materials and methods
Authors:J Ambler
Institution:Clinical Chemistry Department, University Hospital, Queen's Medical Centre, Nottingham, England.
Abstract:The method of isoelectric focusing proteins on cellulose acetate membranes has been improved by the introduction of: (i) plastic-backed membranes, (ii) a commercially available Mini-IEF cell and (iii) sensitive colloidal gold staining. The improved methodology is described with applications for clinical laboratories.
Keywords:
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