Isoelectric focusing on cellulose acetate membranes: an up-date on materials and methods |
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Authors: | J Ambler |
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Institution: | Clinical Chemistry Department, University Hospital, Queen's Medical Centre, Nottingham, England. |
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Abstract: | The method of isoelectric focusing proteins on cellulose acetate membranes has been improved by the introduction of: (i) plastic-backed membranes, (ii) a commercially available Mini-IEF cell and (iii) sensitive colloidal gold staining. The improved methodology is described with applications for clinical laboratories. |
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