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Low intensity infrared laser effects on Escherichia coli cultures and plasmid DNA |
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| Authors: | A. S. Fonseca A. F. Teixeira G. A. Presta M. Geller S. S. Valença F. Paoli |
| Affiliation: | 1. Departamento de Ciências Fisiológicas, Instituto Biomédico, Universidade Federal do Estado do Rio de Janeiro, Rua Frei Caneca, 94, Rio de Janeiro, 20211040, Brazil 2. Departamento de Biofísica e Biometria, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Avenida 28 de Setembro, 87, Vila Isabel, Rio de Janeiro, 20551030, Brazil 3. Centro de Ciências da Saúde, Centro Universitário Serra dos órg?os, Avenida Alberto Torres, 111, Alto, Teresópolis, Rio de Janeiro, 25964004, Brazil 4. Setor de Facomatoses do Servi?o de Genética Clínica do IPPMG, Universidade Federal do Rio de Janeiro, Avenida Brigadeiro Trompowsky, Rio de Janeiro, 21949590, Brazil 5. Instituto de Ciências Biomédicas, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Avenida Carlos Chagas Filho, 373, Bloco J, Ilha do Fundao, Rio de Janeiro, 21941902, Brazil 6. Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, Rua Jose Louren?o Kelmer, s/n—Campus Universitário, S?o Pedro, Juiz de Fora, Minas Gerais, 36036900, Brazil |
| Abstract: | Biostimulative effect of low intensity laser in tissues has been described on a photobiological basis and clinical protocols are recommended for treatment of various diseases. The aim of this work was to evaluate effects of laser exposure on the survival of Escherichia coli cultures and plasmid topological forms. Escherichia coli cultures and plasmids were exposed to infrared laser to study bacterial survival and electrophoretic profile, respectively. Data indicate low intensity infrared laser: (i) had no effect on E. coli wild type, endonuclease IV, exonuclease III, formamidopyrimidine DNA glycosylase/MutM protein and endonuclease III deficient cultures, but decreased the survival of E. coli UvrA protein deficient cultures; (ii) there was no alteration in the electrophoretic profile of plasmids. Exposure to low intensity infrared laser decreases survival of Escherichia coli cultures deficient in nucleotide excision repair of DNA and this effect could depend on fluences, wavelength and tissues conditions. |
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