| 硫醇类荧光探针研究进展 |
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| 引用本文： | 尹伶灵,陈蓁蓁,佟丽丽,徐克花,唐波.硫醇类荧光探针研究进展[J].分析化学,2009,37(7):1073-1081. |
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| 作者姓名： | 尹伶灵 陈蓁蓁 佟丽丽 徐克花 唐波 |
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| 作者单位： | 山东师范大学化学化工与材料科学学院,分子与纳米探针教育部重点实验室,农药、医药中间体清洁生产教育部工程研究中心,济南,250014 |
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| 基金项目： | 国家自然科学基金重大项目，国家自然科学基金，国家重点基础研究发展规划(973计划)，国家杰出青年科学基金，山东省自然科学基金重点项目，教育部高等学校博士学科点专项科研基金 |
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| 摘 要： | 硫醇是生物体中许多蛋白质和小分子的重要组成部分,在细胞的抗氧化系统中具有重要的作用,定量检测硫醇在生物化学和临床化学中具有重要的意义.荧光法由于其具有灵敏度高、能够实现对活体甚至单个细胞的实时可视化示踪的优点,成为目前广泛采用检测细胞内硫醇类物质的一种重要手段.本文引用文献51篇,按荧光探针与巯基作用机理的不同分类,就近年来该领域的研究进展做了比较系统的评述,并展望了此类探针的发展趋势和应用前景.
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| 关 键 词： | 硫醇 荧光探针 综述 |
| 收稿时间： | 27 September 2008 |
Progress on Fluorescent Probes for Thiols |
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| YIN Ling-Ling,CHEN Zhen-Zhen,TONG Li-Li,XU KE-Hua,TANG Bo.Progress on Fluorescent Probes for Thiols[J].Chinese Journal of Analytical Chemistry,2009,37(7):1073-1081. |
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| Authors: | YIN Ling-Ling CHEN Zhen-Zhen TONG Li-Li XU KE-Hua TANG Bo |
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| Institution: | aCollege of Chemistry, Chemical Engineering and Materials Science, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Engineering Research Center of Pesticide and Medicine Intermediate Clean Production, Ministry of Education, Shandong Normal University, Jinan 250014, China |
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| Abstract: | Thiols, which are the components of many proteins and simple molecules, play an important role in the cellular antioxidant defense, and their concentration level is directly linked to many diseases. Therefore, real-time quantitative determination of thiols is of vital significance in biochemistry and clinical chemistry. Fluorescent probe-based detection, which is highly sensitive and specific, is one of the most powerful and attractive tools used in thiols determination, especially in living cell imaging. This review summarizes the recent progress in thiols detection by fluorescent probes. The contents were described and discussed in terms of different reaction mechanisms between fluorescent probes and thiols. Particularly, future studies and prospects were envisioned. |
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| Keywords: | Thiols fluorescent probe review |
| 本文献已被 CNKI 万方数据 ScienceDirect 等数据库收录！ |
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