甲酸钠增敏鲁米诺化学发光机理研究及应用

在表面活性剂十二烷基苯磺酸钠(SDBS)存在下,传统意义上的自由基清除剂甲酸钠反而对铬酸钾一鲁米诺-腺嘌呤化学发光体系表现出了很好的化学发光增敏效果,文章通过化学发光发射光谱,紫外可见光谱及化学反应等手段探讨了这一化学发光体系的增敏机理,结果表明:化学发光体系的增敏是由于反应体系中甲酸钠的存在使原有发光体系中的羟基自由基(OH·)转化为氧自由基(O2·)所致.以此为基础建立了流动注射化学发光法测定腺嘌呤的高灵敏方法,腺嘌呤在2.45×10-11～8.18×10-9 mol·L-1范围内成良好的线性关系(R2=0.999 3),检出限为8.72×10-12 mol·L-1.该法已成功用于测定维生素B4药品中的腺嘌呤的含量,结果令人满意.

Study on the Mechanism of Sodium Formate Enhancing Luminol Chemiluminescence and Its Application in Micellar Medium

In the presence of sodium dodecylbenzene sulfonate (SDBS) surfactant micelles, the signal of K2Cr2O7-luminol-adenine chemiluminescence system was strongly enhanced by sodium formate, which is conventionally considered as free radical scavenger. The present article discussed the sensitizing mechanism of chemiluminescence through the methods of chemiluminescence emission spectra, UV-visible absorption spectra, and chemical reactions. The result indicated that free radical OH·, which existed in the original chemiluminescence system, was transformed into free radical O·2 by the existence of sodium formate. Based on that, a highly sensitive flow injection-chemiluminescence method to determine adenine was established. Under optimal experimental conditions, the concentration of adenine was linear with the chemiluminescence intensity in the range of 8.18×10-9 to 2.45×10-11 mol·L-1. The limit of detection (3σ) was 8.72×10-12 mol·L-1. The method has been successfully applied to the analysis of adenine in VB4 pills and the results are satisfactory.