| 转APX基因烟草抗旱能力研究 |
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| 引用本文： | 韩瑞丽,陆海.转APX基因烟草抗旱能力研究[J].成都大学学报(自然科学版),2007,26(2):93-96,121. |
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| 作者姓名： | 韩瑞丽 陆海 |
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| 作者单位： | 北京林业大学,生物科学与技术学院,北京,100083;北京林业大学,生物科学与技术学院,北京,100083 |
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| 基金项目： | 教育部科学技术研究重点项目(106039)，北京林业大学研究生自选课题(05jj039)资助 |
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| 摘 要： | 用毛白杨中克隆得到的抗坏血酸过氧化物酶(APX)基因,构建植物表达载体pBI121-APX,将pBI121-APX载体用农杆菌介导法转化烟草,成功得到转基因烟.PCR、PCR-Southern的结果表明,APX基因已经成功的整合到转基因烟草基因组中.干旱实验结果表明,与对照相比较,转基因烟草表现出较强的抗旱性.
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| 关 键 词： | 抗坏血酸过氧化物酶 载体构建 抗旱性 转基因烟草 |
| 文章编号： | 1004-5422（2007）02-0093-04 |
| 修稿时间： | 2007-04-15 |
Research of Transgenic Tobacco of APX Gene in Drought Tolerance Analysis |
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| HAN Ruili,LU Hai.Research of Transgenic Tobacco of APX Gene in Drought Tolerance Analysis[J].Journal of Chengdu University (Natural Science),2007,26(2):93-96,121. |
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| Authors: | HAN Ruili LU Hai |
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| Institution: | College of Biological Science and Biotechnology, Beij ng Forestry University, Beijing 100083, China |
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| Abstract: | In this paper,the APX gene cloned from Populus tomentosa was used for the construction of an expression vector:pBI121-APX,it was used for the genetic transformation of tobacco via Agrobacterium mediated method.PCR and PCR-Southern blot analysis indicated that the foreign gene APX had been integrated into the genome of tobacco.Transgenic tobacco showed more resistance under drought stress. |
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| Keywords: | ascorbate peroxidase vector construction drought resistance transgenic tobacco |
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