VACUUM-ULTRAVIOLET PHOTOACOUSTIC SPECTROSCOPY OF BIOLOGICAL MATERIALS USING SYNCHROTRON RADIATION AS A LIGHT SOURCE |
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| Authors: | Takashi Inagaki Atsushi Ito Masatsugu Motosuga Kotaro Hieda Katsumi Kobayashi Hiroshi Maezawa Takashi Ito |
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| Institution: | Physics Department, Osaka Kyoiku University, Tennoji, Osaka 543, Japan;Institute of Physics, College of Arts and Sciences, University of Tokyo, Meguro-Ku, Komaba, Tokyo 153, Japan;Biophysics Laboratory, Department of Physics, Rikkyo (St. Paul's) University, Toshima-ku, Tokyo 171, Japan;Institute of Biological Sciences, University of Tsukuba Ibaraki 305, Japan;Department of Radiology, School of Medicine, Tokai University, Bohseidai, Isehara, Kanagawa259–11, Japan |
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| Abstract: | Abstract— Photoacoustic measurements of biological materials such as DNA, lysozyme, squalene and yeast cells were performed for the first time in the vacuum-UV region above 150 nm using the gas-microphone technique and synchrotron radiation as a light source. The samples studied were in a form of thin film supported on a self-supporting collodion film. The results obtained for the phase of the photoacoustic signal were found consistent with the predictions from the simple one-dimensional theory. The signal amplitudes obtained as a function of the incident wavelength were interpreted as giving the absorptance (the fraction of the incident photon energy dissipated in the sample) spectrum. |
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