鱼腥草中总黄酮提取及其抗过敏活性的研究

 确定鱼腥草中总黄酮的提取工艺条件,并对其抗过敏活性进行研究.经响应曲面优化实验确定了最佳提取条件:提取时间X₁=51.min,液固体积比X₂=49.03:1,提取温度X₃=7.05℃,在此条件下黄酮质量分数为9.03%.对鱼腥草抗过敏功能评价表明:鱼腥草的提取液对透明质酸酶有较强的抑制作用,鱼腥草对透明质酸酶的抑制作用比甜茶强,得到鱼腥草提取液的抗过敏剂量-效应关系,对小鼠的抑制率为35.05%,在剂量为300 mg/kg时能非常显著地抑制组胺诱导的小鼠毛细血管通透性增高.

Study on extracting technology of the flavones in houttuynia cordata thunb and its antiallergic activity

The extract craft conditions of flavones in Houttuynia Cordata Thunb were determined,and its antiallergic activity was studied simultaneously.And the optimal conditions were found by responds surface methodology:extract time is X₁=51.66min,a volume ratio of liquid to solid is X₂=49.03:1,extract temperature is X₃=76.05℃,and under these conditions,the content of flavones is 9.03%.The antiallergic experimentation indicate:Houttuynia Cordata Thunb extract have a strongly inhibition to hyaluronidase,and stronger than Lithocarps Litseifolius Rehd on the inhibition to hyaluronidase,then can get dose-effect relation of Houttuynia Cordata Thunb extract,its inhibition is 35.05% on mice,at the dose of 300mg/kg can very significantly inhibit to mice’s blood capillary penatrativity increased by histamine.