| 螺旋藻糖蛋白的分离纯化及其性质研究 |
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| 引用本文： | 殷钢,刘铮,李琛,刘飞,丁富新,袁乃驹. 螺旋藻糖蛋白的分离纯化及其性质研究[J]. 高等学校化学学报, 1999, 20(4): 565-568 |
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| 作者姓名： | 殷钢 刘铮 李琛 刘飞 丁富新 袁乃驹 |
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| 作者单位： | 清华大学化学工程系, 北京 100084 |
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| 基金项目： | 国家“九·五”科技攻关项目 |
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| 摘 要： | 采用新的分离工艺从螺旋藻中提取得到粗多糖,经环流泡沫分离去除蛋白和SephacrylS-100柱层析纯化后得到一种糖蛋白.经SephadexG-200和凝胶电泳证实所得的糖蛋白为均一组成;由SDS-PAGE测定分子量为130000;红外色谱呈现出典型的多糖吸收峰,含有α-型糖苷连接键;β-消去反应初步表明这是一种存在O-连接的糖蛋白.
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| 关 键 词： | 螺旋藻 糖蛋白 多糖 |
| 收稿时间： | 1998-05-18 |
Isolation, Characterization and Properties of Spirulina Glycoprotein |
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| YIN Gang,LIU Zheng,LI Chen,LIU Fei,DING Fu-Xin,YUAN Nai-Ju. Isolation, Characterization and Properties of Spirulina Glycoprotein[J]. Chemical Research In Chinese Universities, 1999, 20(4): 565-568 |
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| Authors: | YIN Gang LIU Zheng LI Chen LIU Fei DING Fu-Xin YUAN Nai-Ju |
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| Affiliation: | Department of Chemical Engineering, Tsinghua University, Beijing, 100084 |
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| Abstract: | A glycoprotein was isolated from Chinese spirulina. Purification of this glycoprotein was accomplished by foam in a loop bubble column and gel chromatography on Sephacryl S100. The homogeneity of this glycoprotein was proven by gel filtration on Sephadex G200 and SDSPAGE. The molecular weight of the glycoprotein was {130 000}. Characterization of this glycoprotein by IR analysis showed the typical absorption of polysaccharide and indicated the presence of glycoside linkage. Elimination reaction demonstrated the existence of Oglycosidic linkage in this spirulina glycoprotein. |
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| Keywords: | Spirulina Glycoprotein Polysaccharide |
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