Comparison between different sheathless electrospray emitter configurations regarding the performance of nanoscale liquid chromatography-time-of-flight mass spectrometry analysis |
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Authors: | Amirkhani Ardeshir Wetterhall Magnus Nilsson Stefan Danielsson Rolf Bergquist Jonas |
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Institution: | Department of Analytical Chemistry, Institute of Chemistry, Uppsala University, PO Box 599, SE-75124 Uppsala, Sweden. |
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Abstract: | Four different sheathless electrospray ionization (ESI) configurations were investigated for a nano liquid chromatography (LC) system. The studied configurations were: a column with an integrated emitter, with the ESI potential applied before or after the column, and a column with separate emitter, with the ESI voltage applied at a union before the emitter or at the emitter tip. The results indicates that the efficiency of the LC system is rather independent of the configuration when using 95 microm i.d. columns, acetic mobile phase and standard peptides as a sample. Introduction of post column dead volume seems not to be a critical issue at least with flow rates down to 600 nl/min. |
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Keywords: | Electrospray ionization Band broadening Principal component analysis Instrumentation Peptides |
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