Contributions to the determination of histamine rate by measuring out the histamine-orthophthalaldehyde complex in the absorption and fluorescence |
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Authors: | Douabalé Stephy Edgar Dione Mactar Coly Atanasse Tine Alphonse |
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Institution: | Département de Chimie, Laboratoire de Photochimie et d'Analyse, Faculté des Sciences et Techniques, Université Cheikh Anta Diop, Dakar, Senegal. |
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Abstract: | Histamine is an important compound from a physiological point of view, but it is toxic since the absorption of low amounts of histamine can cause abdominal pains accompanied with vomiting. Why we have developed a new method for histamine analysis in order to improve Lerke and Bell method. From the absorption spectra, we showed that the stoichiometry of the complex histamine-orthophthalaldehyde (OPA) is 1:1. From the emission spectra, it was observed that the complex fluorescence is inhibited in acidic medium. In alkaline medium, an exaltation of fluorescence was observed, but the complex histamine-OPA was unstable. Nevertheless, kinetic study showed that good linear correlations between the fluorescence maxima of the formed complex and the histamine concentration could be obtained in this alkaline medium. |
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