| 大熊猫肌酸激酶热变性中活性与构象变化的比较 |
| |
| 引用本文： | 吴海斌,王希成,周海梦,叶掬群.大熊猫肌酸激酶热变性中活性与构象变化的比较[J].清华大学学报(自然科学版),1991(3). |
| |
| 作者姓名： | 吴海斌 王希成 周海梦 叶掬群 |
| |
| 作者单位： | 清华大学生物科学与技术系
(吴海斌，王希成，周海梦)，北京动物园(叶掬群) |
| |
| 摘 要： | 比较了大熊猫肌酸激酶在各种不同温度下热变性时，活性变化与构象变化的速度常数。结果表明在较低温度下，酶的失活速度与构象变化速度相当．而在较高温度下变性时酶的失活速度快于构象变化速度。这表明酶分子的活性部位的微区构象比整个分子的构象具有更大的易变性或柔性。
|
| 关 键 词： | 肌酸激酶 大熊猫 热变性 动力学 |
A Comparison of Rates of Inactivation and Conformational Changes of Panda Creatine Kinase during Thermal Denaturation |
| |
| Wu Haibin, Wang Xicheng, Zhou Haimeng.A Comparison of Rates of Inactivation and Conformational Changes of Panda Creatine Kinase during Thermal Denaturation[J].Journal of Tsinghua University(Science and Technology),1991(3). |
| |
| Authors: | Wu Haibin Wang Xicheng Zhou Haimeng |
| |
| Institution: | Wu Haibin; Wang Xicheng; Zhou Haimeng (Department of Biological Science and Biotechnology Tsinghua University) Ye Juqun (Beijing Zoo) |
| |
| Abstract: | A comparison of rates of inactivation and conformational changes of panda creatine kinase during thermal denaturation at the different temperature was studied. The results show that at lower temperature the inactivation rate of the enzyme is approximately the same with its conformation changes, but at higher temperature the rates of inactivation are faster than its conformational changes. It can be suggested that the conformation of active site of the enzyme is more flexible than that of the whole enzyme molecule. |
| |
| Keywords: | creatine kinase panda thermal denaturation kinetics |
| 本文献已被 CNKI 等数据库收录！ |
