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| Caco-2细胞模型用于毒害污染物转运与吸收研究* | |
| 引用本文： | 王德庆,余应新,钟玉芳,吴明红,盛国英,傅家谟.Caco-2细胞模型用于毒害污染物转运与吸收研究*[J].化学进展,2010,22(4):740-747. |
| 作者姓名： | 王德庆 余应新 钟玉芳 吴明红 盛国英 傅家谟 |
| 作者单位： | (1. 上海大学环境与化学工程学院环境污染与健康研究所 上海 200444；2. 中国科学院广州地球化学研究所有机地球化学国家重点实验室 广东省环境资源利用与保护重点实验室 广州 510640) |
| 基金项目： | 国家自然科学基金重点项目;国家自然科学青年基金项目;上海市重点学科 |
| 摘 要： | Caco-2 细胞来源于人类结肠癌细胞，其单层细胞模型常用于体外模拟肠道上皮细胞转运与吸收外源性物质的研究，并被广泛地应用于毒理学研究等方面。本文简要介绍了Caco-2 单层细胞模型的培养、细胞特性及常用功能指标；详细综述了环境毒害污染物在Caco-2细胞中转运与吸收机制以及污染物细胞毒性对其转运与吸收的影响，并对Caco-2 单层细胞模型在环境毒害污染物的人体健康风险评估中的应用进行了展望。 |
| 关 键 词： | Caco-2细胞 体外实验 转运与吸收 毒害污染物 生物利用度 环境风险评估 |
| 收稿时间： | 2009-05-18 |
| 修稿时间： | 2009-07-06 |
Application of Caco-2 Cell Model to Transportation and Absorption of Toxic Substances |
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| Wang Deqing,Yu Yingxin,Zhong Yufang,Wu Minghong,Sheng Guoying,Fu Jiamo.Application of Caco-2 Cell Model to Transportation and Absorption of Toxic Substances[J].Progress in Chemistry,2010,22(4):740-747. | |
| Authors: | Wang Deqing Yu Yingxin Zhong Yufang Wu Minghong Sheng Guoying Fu Jiamo |
| Institution: | (1.Institute of Environmental Pollution and Health, School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China；2. State Key Laboratory of Organic Geochemistry, Guangdong Key Laboratory of Environmental Resources Utilization and Protection, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China) |
| Abstract: | The Caco-2 cell monolayer, isolated from human colon carcinoma, has been wildly used in toxicology study and used as a tool to study the intestinal uptake and transport processes of xenobiotic. We introduces the culture conditions, the basic characteristics and functional parameters of Caco-2 cell monolayer model in brief. The paper also discussed the application of Caco-2 cell monolayer model in studying the mechanism of intestinal transportation and absorption for environmental toxic substances and toxic effects of these substances on their intestinal transportation; absorption in detail. In addition, the application of Caco-2 cells in human health risk assessment for environmental toxic substances is prospected.
Contents 1 Introduction 2 Caco-2 cell monolayer model 3 Transportation and absorption of environmental toxic substances 3.1 Transportation and absorption of heavy metals 3.2 Transportation and absorption of organic pollutants 4 Perspectives |
| Keywords: | Caco-2 cells in vitro test transportation and absorption toxic substances bioavailability environmental risk assessment |
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