Structural analysis of the N-linked oligosaccharides from human urinary trypsin inhibitor. |
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Authors: | H Toyoda T Ikei Y Demachi T Toida T Imanari |
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Affiliation: | Faculty of Pharmaceutical Sciences, Chiba University, Japan. |
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Abstract: | The N-linked oligosaccharides from human urinary trypsin inhibitor were purified and their structures were investigated by compositional analysis, the two-dimensional sugar map method and 500 MHz 1H-NMR. The results revealed that they were composed of disialosyl, monosialosyl and asialosyl oligosaccharides, which have the common biantennary core structure; Gal1-4GlcNAc1-2Man1-3(Gal1-4GlcNAc1-2Man1-6)M an1-4GlcNAc1-4GlcNAc. |
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