Development of a large-volume, membrane-based injection system for gradient elution micro-liquid chromatography |
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Authors: | Murata Kaoru Mano Nariyasu Asakawa Naoki |
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Affiliation: | Analytical Research Laboratories, Eisai Co., Ltd., 5-1-3 Tokodai, Tsukuba, Ibaraki 300-2635, Japan. k-murata@hhc.eisai.co.jp |
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Abstract: | A membrane unit which can be used to inject a large volume of sample solution was developed to facilitate reproducible and accurate gradient elution in micro-high-performance liquid chromatography (micro-HPLC). Since the membrane unit has a very low void volume, it facilitates the effective concentration of analytes in large sample volumes. Gradient elution micro-HPLC with the membrane unit allowed the efficient separation of n-alkyl benzoates, used as test samples, in a short time without marked gradient delay. In this study, the membrane unit could be loaded with up to 50 microg of n-hexyl benzoate, and more than 500 microL of sample solution could be applied. In about 50 chromatographic runs, the relative standard deviation (RSD) of the relative retention time of n-hexyl benzoate with respect to methyl benzoate was 0.530%. |
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