Polyphenolic characterization and evaluation of multimode antioxidant,cytotoxic, biocompatibility and antimicrobial potential of selected ethno-medicinal plant extracts |
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| Institution: | 1. Department of Pharmacy, Quaid-i-Azam University, Islamabad 45320, Pakistan;2. Shifa College of Pharmaceutical Sciences, Shifa Tameer-e-Millat University, Jaffer Khan Jamali Road, H-8/4, Islamabad, Pakistan;3. Department of Biology, Science Unit, Deanship of Educational Services, Qassim University, Buraydah 51452, Saudi Arabia;4. BreathMAT Lab, Pakistan Institute of Nuclear Science and Technology, Islamabad, Pakistan;5. Department of Biochemistry, Shah Abdul Latif University, Khairpur 66020, Sindh, Pakistan;6. College of Pharmacy, Gachon University, No. 191, Hambakmoero, Yeonsu-gu, Incheon 21936, Republic of Korea |
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| Abstract: | IntroductionScientific evidence about biological profile of natural products can support their traditional uses. The current work was aimed to assess phytochemical and biological profile of nine medicinal plants collected from Herbalists.MethodsExtracts prepared in different solvents were subjected to phytochemical, antioxidant, enzyme inhibitory, cytotoxic, and antimicrobial activities. Reverse phase-high performance liquid chromatography (RP-HPLC) analysis was performed for the quantification of polyphenols.ResultsResults showed methanol extract (M) being potent as compared to others. Gentian lutea M showed maximum extract recovery (15.00 ± 0.11 % w/w) and TFC (30.82 ± 0.21 μg QE/mg extract). Nigella sativa M displayed highest TPC (44.99 ± 0.43 μg GAE/mg extract) and TAC (334.72 ± 0.35 μg AAE/ mg extract). Results showed noteworthy quantities of vanillic acid, rutin, kaempferol, emodin in ethyl acetate (EA) and methanol (M) extracts of plants assessed by RP-HPLC. Gentisic acid was highest (11.75 µg/mg extract) in T. arjuna M extract. Similarly, maximum %FRSA (82.28 ± 0.03 %) and TRP (160.40 ± 0.38 μg AAE/ mg extract) were depicted by Terminalia chebula and Chamomilla recutita, respectively. Moreover, Mentha longifolia and G. lutea M demonstrated noteworthy (p < 0.05) antibacterial activity against Staphylococcus aureus (14 ± 0.7 mm) and Klebsiella pneumoniae (12 ± 0.3 mm), respectively. Curcuma amada, C. recutita, Murraya koenigii and G. lutea M had significant α-glucosidase activity. Another good solvent for extraction was ethyl acetate (EA), whose extracts were secondary to methanol in producing significant biological profile. For example, EA of N. sativa (TPC: 1.46 ± 0.45 µg GAE/ mg extract), G. lutea (TRP: 160.33 ± 0.52 μg AAE/mg extract: ZOI of 12 ± 0.5 mm in K. pneumoniae) and Mormodica charantia (α-amylase inhibition: 39.5 ± 0.10 %) showed significant bioactivities. All extracts displayed mild antifungal protein kinase inhibition activities and were significantly (greater than80 %: p < 0.05) cytotoxic to brine shrimps with negligible hemolytic activity.ConclusionBriefly, variable polarity solvent extracts of studied plants will be processed for isolation of antioxidant, cytotoxic, carbohydrate enzyme inhibitory and antibacterial compounds. |
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| Keywords: | Natural products Phytoconstituents Antioxidant Antibacterial Antidiabetic potential Cytotoxic activity EA"} {"#name":"keyword" "$":{"id":"k0040"} "$$":[{"#name":"text" "_":"Ethyl acetate M"} {"#name":"keyword" "$":{"id":"k0050"} "$$":[{"#name":"text" "_":"Methanol A"} {"#name":"keyword" "$":{"id":"k0060"} "$$":[{"#name":"text" "_":"Aqueous TPC"} {"#name":"keyword" "$":{"id":"k0070"} "$$":[{"#name":"text" "_":"Total phenolic contents TFC"} {"#name":"keyword" "$":{"id":"k0080"} "$$":[{"#name":"text" "_":"Total flavonoid contents GAE"} {"#name":"keyword" "$":{"id":"k0090"} "$$":[{"#name":"text" "_":"Gallic acid equivalent QE"} {"#name":"keyword" "$":{"id":"k0100"} "$$":[{"#name":"text" "_":"Quercetin equivalent %FRSA"} {"#name":"keyword" "$":{"id":"k0110"} "$$":[{"#name":"text" "_":"Free radical scavenging activity DPPH"} {"#name":"keyword" "$":{"id":"k0120"} "$$":[{"#name":"text" "_":"2 2-Diphenyl-1-picrylhydrazyl TAC"} {"#name":"keyword" "$":{"id":"k0130"} "$$":[{"#name":"text" "_":"Total antioxidant capacity AAE"} {"#name":"keyword" "$":{"id":"k0140"} "$$":[{"#name":"text" "_":"Ascorbic acid equivalent TRP"} {"#name":"keyword" "$":{"id":"k0150"} "$$":[{"#name":"text" "_":"Total reducing power DMSO"} {"#name":"keyword" "$":{"id":"k0160"} "$$":[{"#name":"text" "_":"Dimethyl sulfoxide MIC"} {"#name":"keyword" "$":{"id":"k0170"} "$$":[{"#name":"text" "_":"Minimum inhibitory concentration 50% Inhibitory concentration Lethal concentration causing 50% mortality ZOI"} {"#name":"keyword" "$":{"id":"k0200"} "$$":[{"#name":"text" "_":"Zone of inhibition BZ"} {"#name":"keyword" "$":{"id":"k0210"} "$$":[{"#name":"text" "_":"Bald zone CZ"} {"#name":"keyword" "$":{"id":"k0220"} "$$":[{"#name":"text" "_":"Clear zone |
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