Optimization of extraction process of Dioscorea nipponica Makino saponins and their UPLC-QTOF-MS profiling,antioxidant, antibacterial and anti- inflammatory activities |
| |
| Affiliation: | 1. Key Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, No 120 Dongling Road Shenhe Dist., 110866 Shenyang, Liaoning Prov., People’s Republic of China;2. China Medical University-The Queen’s University of Belfast Joint College, China Medical University, No.77 Puhe Road, 110122 Shenyang, Liaoning Prov., People’s Republic of China;3. School of Pharmacy, China Medical University, No.77 Puhe Road, 110122 Shenyang, Liaoning Prov., People’s Republic of China |
| |
| Abstract: | Dioscorea nipponica Makino exhibits many biological activities, including relieving cough, eliminating phlegm and preventing asthma. The present study extensively evaluated the extraction process, major components, antioxidant, antibacterial and anti-inflammatory activities of total saponins extraction from Dioscorea nipponica Makino. In this study, the optimal extraction process of total saponins extract was optimized by single-factor test and response surface methodology as follows: extraction time 25 min, ethanol concentration 50 % and liquid to material ratio 55:1 ml/g, and the extraction rate was 1.72 %. Eighteen components were initially analyzed by UPLC-QTOF-MS method. Although total saponins extract exhibited mild antibacterial activities against Escherichia coli, Salmonella, Staphylococcus aureus and Streptococcus, and antioxidant activities against ferric-ion, ABTS and DPPH radicals, the perfect anti-inflammatory activity of TSE was demonstrated by significantly reducing the content of NO and the phagocytic activity in LPS induced RAW 264.7 cells, which provided a theoretical basis for the research and development of new anti-inflammatory Chinese medicine. |
| |
| Keywords: | Saponins Extraction process Effective constituents Anti-inflammatory activity BBD" },{" #name" :" keyword" ," $" :{" id" :" k0035" }," $$" :[{" #name" :" text" ," _" :" Box-Behnken design LPS" },{" #name" :" keyword" ," $" :{" id" :" k0045" }," $$" :[{" #name" :" text" ," _" :" lipopolysaccharide TSC" },{" #name" :" keyword" ," $" :{" id" :" k0055" }," $$" :[{" #name" :" text" ," _" :" total saponins content TSE" },{" #name" :" keyword" ," $" :{" id" :" k0065" }," $$" :[{" #name" :" text" ," _" :" total saponins extract |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
