A spectrophotometric method for quantitative determination of lactulose in pharmaceutical preparations |
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| Authors: | Mir Azam Khan Zafar Iqbal Mohammad Rasul Jan Jasmin Shah Waqar Ahmad Zia Ul Haq Obaidullah |
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| Institution: | (1) Department of Chemistry, University of Peshawar, Peshawar-, 25120, Pakistan;(2) Department of Pharmacy, University of Peshawar, Peshawar-, 25120, Pakistan;(3) Department of Pharmacy, Malakand University, Peshawar-, 25120, Pakistan;(4) Libra Pvt Ltd. Industrial Estate, Peshawar, Pakistan;(5) Drug Control Administration, Ministry of Health, Peshawar, Pakistan |
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| Abstract: | A simple spectrophotometric assay for the quantification of lactulose in pharmaceutical preparations was developed. The method
is based on hydrolysis of lactulose under acidic conditions. The hydrolyzed product reacts with resorcinol, giving absorption
peaks at 398 and 480 nm. Both absorption wavelengths can be used for the determination of lactulose. The limit of detection
of lactulose at 398 nm and 480 nm was 0.075 μg mL−1 and 0.65 μg mL−1, respectively. The calibration was linear in the range of 5–25 μg mL−1. Analytical conditions were optimized, and the method was validated for analysis of pharmaceutical preparations. The determined
amount of lactulose was found to be in good agreement with labeled claims in commercial products. The proposed method is economical,
convenient, and suitable for the quantification of lactulose in pharmaceutical preparations.
The text was submitted by the authors in English. |
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