Cyanogen bromide cleavage generates fragments suitable for expressed protein and glycoprotein ligation |
| |
| Authors: | Macmillan Derek Arham Lubna |
| |
| Affiliation: | School of Chemistry, University of Edinburgh, Kings Buildings, West Mains Road, Edinburgh, Scotland, UK. derek.macmillan@ed.ac.uk |
| |
| Abstract: | Herein cyanogen bromide is employed for the efficient production of N-terminal cysteine containing protein fragments for expressed protein ligation (EPL) from polyhistidine-tagged precursors. We provide three examples of efficient CNBr cleavage of fragments of the glycoprotein erythropoietin that can be ligated with peptides or glycopeptide mimetics potentially giving rise to semisynthetic glycoprotein therapeutics. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
