Screening approach for chiral separation of pharmaceuticals part II. Reversed-phase liquid chromatography |
| |
Authors: | Perrin C Matthijs N Mangelings D Granier-Loyaux C Maftouh M Massart D L Heyden Y Vander |
| |
Affiliation: | Department of Pharmaceutical and Biomedical Analysis, Pharmaceutical Institute, Vrije Universiteit Brussel, Belgium. |
| |
Abstract: | A screening strategy for the rapid separation of drug enantiomers by reversed-phase liquid chromatography was developed using three cellulose/amylose stationary phases. The key point to achieve enantioselectivity is the control of the compound ionisation. Only two mobile phases, i.e. an acidic phosphate buffer (pH 2.0) containing a chaotropic salt (KPF6) and a borate buffer (pH 9.0) mixed with acetonitrile, are used in the proposed strategy. This strategy was successfully applied to a set of 37 diverse chiral pharmaceuticals. Satisfactory enantioselectivity was achieved for 89% of them. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|