Gas Chromatographic Method for Routine Determination of Oleanolic and Ursolic Acids in Medicinal Plants |
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| Authors: | G Janicsák Veres Kállai Máthé |
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| Institution: | (1) Institute of Ecology and Botany of the Hungarian Academy of Sciences, Alkotmány út 2–4., 2163 Vácrátót, Hungary;(2) Department of Pharmacognosy, University of Szeged, Eötvös utca 6., 6720 Szeged, Hungary;(3) Institute of General and Analytical Chemistry, Chemical Instrumentation centre of the National Scientific Research Fund, Budapest University of Technology and Economics, Szt. Gellért tér 4., 1111 Budapest, Hungary |
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| Abstract: | Summary A rapid and simple gas chromatographic method has been established for routine analysis of free oleanolic and ursolic acids in dried samples of medicinal herbs. Soxhlet extraction of triterpenes was followed by solid-phase extraction (SPE). Amounts of the compounds were measured by gas chromatography after silylation of the purified samples. Experiments were performed to establish the optimum conditions (e.g. solvent, and mode and duration of extraction) for calibration curve linearity, sensitivity, reproducibility, and recovery. The conditions used for derivatization and gas chromatographic analysis resulted in an improvement on literature data. The method devised enables accurate routine measurement of many samples in quite a short time (e.g. for chemotaxonomical screening, or quality control of herbal drugs). The practical application of the method was illustrated on five Lamiaceae species. |
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| Keywords: | Gas chromatography Solid-phase extraction Derivatization Oleanolic and ursolic acid Medicinal plants |
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