Quantitative Monitoring and Visualization of Hydrogen Sulfide In Vivo Using a Luminescent Probe Based on a Ruthenium(II) Complex |
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| Authors: | Zhongbo Du Dr Bo Song Dr Wenzhu Zhang Chengchen Duan Dr Yong‐Lei Wang Chaolong Liu Dr Run Zhang Prof Jingli Yuan |
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| Institution: | 1. State Key Laboratory of Fine Chemicals, School of Chemistry, Dalian University of Technology, Dalian, China;2. Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St. Lucia, Australia;3. Department of Chemistry, Stanford University, Stanford, CA, USA |
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| Abstract: | Development of novel bioanalytical methods for monitoring of H2S is key toward understanding the physiological and pathological functions of this gasotransmitter in live organisms. A ruthenium(II)‐complex‐based luminescence probe, Ru‐MDB (MDB: 4’‐methyl‐2,2’‐bipyridine]‐4‐yl)methyl 2‐((2,4‐dinitrophenyl)thio)benzoate), was developed by introducing a new H2S responsive masking moiety to a red‐emitting RuII luminophore. Cleavage of this masking group by a H2S‐triggered reaction leads to a luminescence “off–on” response. The long‐lived emissions of Ru‐MDB and its reaction product with H2S allowed quantitative detection of H2S in autofluorescence‐rich human sera and adult zebrafish organs using the time‐gated luminescence mode. Ru‐MDB exhibits red emission, a large Stokes shift, high specificity and sensitivity for H2S detection, and low cytotoxicity, which enables imaging and flow cytometry analysis of lysosomal H2S generation in live inflamed cells under drug stimulation. Monitoring of H2S in live Daphnia magna, zebrafish embryos, adult zebrafish, and mice, was conducted by in vivo imaging using Ru‐MDB as a probe. |
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| Keywords: | analytical methods hydrogen sulfide in vivo sensing ruthenium complexes zebrafish models |
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