Valency‐Controlled Framework Nucleic Acid Signal Amplifiers |
| |
| Authors: | Dr. Qi Liu Dr. Zhilei Ge Dr. Xiuhai Mao Dr. Guobao Zhou Prof. Xiaolei Zuo Dr. Juwen Shen Dr. Jiye Shi Prof. Jiang Li Prof. Lihua Wang Prof. Xiaoqing Chen Prof. Chunhai Fan |
| |
| Affiliation: | 1. Institute of Molecular Medicine, Renji Hospital, School of Medicine and School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, Shanghai, China;2. College of Chemistry and Chemical Engineering, Central South University, Changsha, China;3. Division of Physical Biology and Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, China;4. Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai, China;5. UCB Pharma, Slough, UK |
| |
| Abstract: | Weak ligand–receptor recognition events are often amplified by recruiting multiple regulatory biomolecules to the action site in biological systems. However, signal amplification in in vitro biomimetic systems generally lack the spatiotemporal regulation in vivo. Herein we report a framework nucleic acid (FNA)‐programmed strategy to develop valence‐controlled signal amplifiers with high modularity for ultrasensitive biosensing. We demonstrated that the FNA‐programmed signal amplifiers could recruit nucleic acids, proteins, and inorganic nanoparticles in a stoichiometric manner. The valence‐controlled signal amplifier enhanced the quantification ability of electrochemical biosensors, and enabled ultrasensitive detection of tumor‐relevant circulating free DNA (cfDNA) with sensitivity enhancement of 3–5 orders of magnitude and improved dynamic range. |
| |
| Keywords: | biosensors cfDNA DNA nanostructure framework nucleic acids valence-engineering |
|
|
