Supramolecular Reassembly of Self‐Exfoliated Ionic Covalent Organic Nanosheets for Label‐Free Detection of Double‐Stranded DNA |
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| Authors: | Arindam Mal Dr. Rakesh K. Mishra Dr. Vakayil K. Praveen M. Abdul Khayum Dr. Rahul Banerjee Prof. Dr. Ayyappanpillai Ajayaghosh |
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| Affiliation: | 1. Photosciences and Photonics Section, Chemical Sciences and Technology Division and Academy of Scientific and Innovative Research (AcSIR), CSIR-National Institute for Interdisciplinary Science and Technology (CSIR-NIIST), Thiruvananthapuram, India;2. Physical/Materials Chemistry Division and Academy of Scientific and Innovative Research (AcSIR), CSIR-National Chemical Laboratory (CSIR-NCL), Pune, India |
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| Abstract: | Ionic covalent organic nanosheets (iCONs), a member of the two‐dimensional (2D) nanomaterials family, offer a unique functional platform for a wide range of applications. Herein, we explore the potential of an ethidium bromide (EB)‐based covalent organic framework ( EB‐TFP ) that self‐exfoliates in water resulting in 2D ionic covalent organic nanosheets ( EB‐TFP‐iCONs ) for the selective detection of double‐stranded DNA (dsDNA). In an aqueous medium, the self‐exfoliated EB‐TFP‐iCONs reassemble in the presence of dsDNA resulting in hybrid EB‐TFP‐iCONs‐DNA crystalline nanosheets with enhanced fluorescence at 600 nm. Detailed steady‐state and time‐resolved emission studies revealed that the reassembly phenomenon was highly selective for dsDNA when compared to single‐stranded DNA (ssDNA), which allowed us to use the EB‐TFP‐iCONs as a 2D fluorescent platform for the label‐free detection of complementary DNA strands. |
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| Keywords: | DNA covalent organic framework ionic assembly ionic covalent organic nanosheets label-free detection |
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