A Multi‐signal Fluorescent Probe with Multiple Binding Sites for Simultaneous Sensing of Cysteine,Homocysteine, and Glutathione |
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| Authors: | Guo‐xing Yin Ting‐ting Niu Ya‐bing Gan Ting Yu Dr Peng Yin Prof Hai‐min Chen Prof You‐yu Zhang Prof Hai‐tao Li Prof Shou‐zhuo Yao |
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| Institution: | 1. Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, China;2. Key Laboratory of Marine Biotechnology of Zhejiang Province, Ningbo University, Zhejiang, P. R. China |
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| Abstract: | A novel fluorescent probe was developed by integrating chlorinated coumarin and benzothiazolylacetonitrile and exploited for simultaneous detection of cysteine (Cys), homocysteine (Hcy), and glutathione (GSH). Featuring four binding sites and different reaction mechanisms for different biothiols, this probe exhibited rapid fluorescence turn‐on for distinguishing Cys, Hcy, and GSH with 108‐, 128‐, 30‐fold fluorescence increases at 457, 559, 529 nm, respectively, across different excitation wavelengths. Furthermore, the probe was successfully applied to the fluorescence imaging of endogenous Cys and GSH and exogenous Cys, Hcy, and GSH in living cells. |
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| Keywords: | biosensors cysteine fluorescent probes glutathione thiols |
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