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A Cell‐Targeted Non‐Cytotoxic Fluorescent Nanogel Thermometer Created with an Imidazolium‐Containing Cationic Radical Initiator |
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| Authors: | Dr Seiichi Uchiyama Dr Toshikazu Tsuji Kyoko Kawamoto Dr Kentaro Okano Eiko Fukatsu Takahiro Noro Kumiko Ikado Sayuri Yamada Yuka Shibata Dr Teruyuki Hayashi Dr Noriko Inada Dr Masaru Kato Dr Hideki Koizumi Prof Hidetoshi Tokuyama |
| Institution: | 1. Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan;2. Central Laboratories for Key Technologies, KIRIN Company Limited, Yokohama, Kanagawa, Japan;3. Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Miyagi, Japan;4. Department of Chemical Science and Engineering, Kobe University, Kobe, Hyogo, Japan;5. The Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara, Japan;6. Department of Nutrition, Koshien University, Takarazuka, Hyogo, Japan |
| Abstract: | A cationic fluorescent nanogel thermometer based on thermo‐responsive N‐isopropylacrylamide and environment‐sensitive benzothiadiazole was developed with a new azo compound bearing imidazolium rings as the first cationic radical initiator. This cationic fluorescent nanogel thermometer showed an excellent ability to enter live mammalian cells in a short incubation period (10 min), a high sensitivity to temperature variations in live cells (temperature resolution of 0.02–0.84 °C in the range 20–40 °C), and remarkable non‐cytotoxicity, which permitted ordinary cell proliferation and even differentiation of primary cultured cells. |
| Keywords: | fluorescence spectroscopy fluorescent probes imaging agents nanoparticles sensors |