Reusability of entrapped cells of Pseudomonas diminuta for production of 7-aminocephalosporanic acid |
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Authors: | V K Nigam S Kundu P Ghosh |
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Institution: | (1) Birla Institute of Scientific Research, Statue Circle, Jaipur-302001, Rajasthan;(2) School of Biochemical Engineering, Institute of Technology, Banaras Hindu University, Varanasi-221005, (India) |
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Abstract: | Entrapped cells of P. diminuta were used for the production of 7-aminocephalosporanic acid (7-ACA), a key intermediate required for the production of most
of the clinically used cephalosporin derivatives, i.e., semisynthetic cephalosporins. The repeated batch production of 7-ACA
with entrapped cells of P. diminuta in different carriers were carried out for six cycles at optimal conditions. It was found that 33%, 38%, and 47% of activity
was lost with chitosan, gelatin, and agar, respectively as immobilizing supports after the sixth cycle of operation. |
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Keywords: | 7-ACA CPC acylase GL-7-ACA GL-7-ACA acylase immobilization repeated batch production |
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