The role of oxygen in the reduction of tetrazolium salts with nadh mediated by 5-methyl phenazonium methyl sulfate. An EPR and voltammetric study |
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| Authors: | P Carloni L Greci E Maurelli P Stipa M Wozniak G Marrosu R Petrucci A Trazza |
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| Institution: | 1. Dipartimento di Scienze dei Materiali e della Terra, Università di Ancona, Via Brecce Bianche, I-60131, Ancona, Italy
2. Dipartimento di Ingegneria Chimica, dei Materiali, delle Materie Prime e Metallurgia, Sezione Chimica, Università di Roma “La Sapienza”, Via del Castro Laurenziano 7, I-00161, Roma, Italy
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| Abstract: | The reduction of tetrazolium salts (namely INT, MTT and BNT) with NADH in the presence of catalytic amounts of PMS (5-methylphenazonium
methylsulfate) was studied, and the influence of oxygen on the system was considered. The redox potentials of all the investigated
compounds, the kinetic measurements, as well as the application of the Marcus theory confirmed that PMS and tetrazolium ions
are reduced by NADH through an inner-sphere mechanism. The EPR investigations led to the detection of all possible radical
species coming from PMS and tetrazolium ions and are in agreement with a mechanism which excludes any role of oxygen in the
system NADH/PMS/tetrazolium for the formation of formazan. |
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