| 应用停流时间分辨光谱研究氧合血红蛋白与过氧化氢反应中间体的动力学和机理 |
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| 引用本文： | 王瑞勇,蔡汝秀,林智信. 应用停流时间分辨光谱研究氧合血红蛋白与过氧化氢反应中间体的动力学和机理[J]. 高等学校化学学报, 2004, 25(2): 267-269 |
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| 作者姓名： | 王瑞勇 蔡汝秀 林智信 |
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| 作者单位： | 武汉大学化学与分子科学学院, 武汉 430072 |
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| 基金项目： | 国家自然科学基金 (批准号 :2 0 2 75 0 2 7)资助 |
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| 摘 要： | 研究血红蛋白与H2O2的相互作用过程及其反应机理具有重要的意义，关于H2O2与血红蛋白相互作用的研究已有报道，但观点不尽相同，结论各异，快速混合停流光谱是研究快速反应动力学的主要手段，在解释化学和生化反应机理方面发挥了重要的作用，本文利用停流时间分辨光谱研究了氧
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| 关 键 词： | 停流时间分辨光谱 氧合血红蛋白 过氧化氢 机理 |
| 文章编号： | 0251-0790(2004)02-0267-03 |
| 收稿时间： | 2003-02-21 |
Studies on the Kinetics and Mechanism of Intermediates of Rexaction between Oxyhemoglobin and H2O2 Using Stopped-flow Time-resolved Spectrophotometry |
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| WANG Rui-Yong,CAI Ru-Xiu ,LIN Zhi-Xin. Studies on the Kinetics and Mechanism of Intermediates of Rexaction between Oxyhemoglobin and H2O2 Using Stopped-flow Time-resolved Spectrophotometry[J]. Chemical Research In Chinese Universities, 2004, 25(2): 267-269 |
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| Authors: | WANG Rui-Yong CAI Ru-Xiu LIN Zhi-Xin |
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| Affiliation: | Department of Chemistry, Wuhan University, Wuhan 430072, China |
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| Abstract: | Interaction between oxyhemoglobin and H_2O_2 was studied by the aid of stopped-flow time-resolved spectrophotometry. The consecutive reaction kinetics of oxyhemoglobin with H_2O_2 suggested the presence of an intermediate species. The influence of pH, concentration of H_2O_2, and temperature on the pseudo-first order rate constants was studied. The kinetic experiments performed at different temperatures allowed to calculate the activation energy. A reasonable mechanism was also proposed. |
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| Keywords: | Stopped-flow time-resolved spectrophotometry Oxyhemoglobin Hydrogen peroxide Mechanism |
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