一种新的荧光素类荧光探针的设计合成及其对血清中组氨酸的选择性标记

组氨酸是人类必需的一种氨基酸 .它含有高反应活性的咪唑环 ,并在生物体系中金属离子的传输和神经递质的传送等方面起着重要的作用 [1,2 ] .所以 ,生物体液中组氨酸的选择性测定对生物化学的研究具有重要意义 .然而 ,由于各种氨基酸在结构及性质上的相似性 ,选择性测定单独的氨基酸常常需要借助如高效液相色谱和毛细管电泳等一些分离技术才能完成 .各种化合物的官能团反应可为一些高选择性的分子识别提供基础[3] .目前各种主体分子 ,包括荧光纳米探针和分子印迹聚合物等在生物分子的选择性识别方面发挥着重要的作用[4～ 6 ] .环氧氯丙烷作为…

Design and Synthesis of a Novel Fluorescein-based Fluorescent Probe for Labeling of Histidine in Human Serum

The synthesis of a novel long-wavelength fluorescent probe, 3-epoxypropoxy fluorescein, and its properties for labeling of histidine are briefly described in this communication. The probe is highly selective for histidine, and other amino acids with a concentration of 1 000 times higher than that of histidine did not show noticeable interferences. As an application of this probe, fluorescent labeling of histidine in human serum was attempted and the obtained results were in agreement with those given by using histidine-nickel complex adsorptive voltammetry, both of which were within the normal range of the results reported in literatures.