| Cholesterol oxidase biosensor based on a glassy carbon electrode modified with Nafion and methyl viologen |
| |
| 引用本文： | JIN,Li-Tong ZHAO,Gui-Zhu FANG,Yu-ZhiDepartment of Chemistry,East China Normal University,Shanghai 200062,China. Cholesterol oxidase biosensor based on a glassy carbon electrode modified with Nafion and methyl viologen[J]. 中国化学, 1994, 12(4): 343-347. DOI: 10.1002/cjoc.19940120408 |
| |
| 作者姓名： | JIN Li-Tong ZHAO Gui-Zhu FANG Yu-ZhiDepartment of Chemistry East China Normal University Shanghai 200062 China |
| |
| 作者单位： | JIN，Li-Tong ZHAO，Gui-Zhu FANG，Yu-ZhiDepartment of Chemistry，East China Normal University，Shanghai 200062，China |
| |
| 摘 要： | Cholesterol oxidase biosensor has been constructed by using bovine serum albumin and glutaraldehyde as cross linker to immobilize cholesterol oxidase and cholesterol esterase on a glassy carbon electrode modified with Nafion and methyl viologen. The biosensor has been used to determine total cholesterol in blood. The linear range of the determination is 2.5×10~7 to 1.0×10-4 mol/L. The detection limit is about 5.0×10~8 mol/L. The response time is 12 s. This biosensor has the advantage of high selectivity, sensitivity and short response time.
|
Cholesterol oxidase biosensor based on a glassy carbon electrode modified with Nafion and methyl viologen |
| |
| JIN,Li-Tong ZHAO,Gui-Zhu FANG,Yu-Zhi. Cholesterol oxidase biosensor based on a glassy carbon electrode modified with Nafion and methyl viologen[J]. Chinese Journal of Chemistry, 1994, 12(4): 343-347. DOI: 10.1002/cjoc.19940120408 |
| |
| Authors: | JIN Li-Tong ZHAO Gui-Zhu FANG Yu-Zhi |
| |
| Affiliation: | JIN,Li-Tong ZHAO,Gui-Zhu FANG,Yu-ZhiDepartment of Chemistry,East China Normal University,Shanghai 200062,China |
| |
| Abstract: | Cholesterol oxidase biosensor has been constructed by using bovine serum albumin and glutaraldehyde as cross linker to immobilize cholesterol oxidase and cholesterol esterase on a glassy carbon electrode modified with Nafion and methyl viologen. The biosensor has been used to determine total cholesterol in blood. The linear range of the determination is 2.5×10?7 to 1.0×10?4 mol/L. The detection limit is about 5.0×10?8 mol/L. The response time is 12 s. This biosensor has the advantage of high selectivity, sensitivity and short response time. |
| |
| Keywords: | Biosensor cholesterol oxidase enzyme electrode. |
| 本文献已被 CNKI 等数据库收录！ |
