Immobilization on chitosan of a thermophilic βglycosidase expressed inSaccharomyces cerevisiae |
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Authors: | Sabato D’Auria Fernando Pellino Francesco La Cara Roberto Barone Mosè Rossi Roberto Nucci |
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Affiliation: | (1) Istituto di Biochimica delle Proteine ed Enzimologia, C. N. R., Via Marconi 10, 80125 Naples, Italy |
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Abstract: | ASulfolobus solfataricus β-glycosidase expressed inSaccharomyces cerevisiae (Sβgly) was immobilized on chitosan activated with glutaraldehyde. The yield of immobilization was evaluated as 80%. Compared to the free β-glycosidase, the immobilized enzyme showed a similar pH optimum (pH = 7.0), the same increasing activity up to 80°C, improved thermostability, and no inhibition by glucose. Functional studies pointed out that the kinetic constant values for both enzymes were comparable. A bioreactor, assembled with the immobilized Sβgly, was used for glucose production. The values of cellobiose conversion increased on increasing residence time in the bioreactor, following a nonlinear trend. However, the highest glucose production/ min was obtained at a flow of 0.5 mL/min. |
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Keywords: | β -Glycosidase immobilized support chitosan glutaraldehyde coupling agent immobilized β -glycosidase properties glucose production cellobiose hydrolysis |
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