Isolation and characterization of free haem from the shell gland of quail and hen |
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| Authors: | Gorchein A Lord G Lim C K |
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| Institution: | Department of Biological Sciences, Birkbeck, University of London, Malet Street, London, WC1E 7HX, UK. abel.gorchein@talktalk.net |
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| Abstract: | Free haem was isolated from the shell gland of the quail, Coturnix coturnix japonica, and of the fowl, Galinus domesticus, and characterized by HPLC‐ESI‐MS/MS. Quantification by HPLC gave values of 1.17–1.40 nmol/mg quail shell gland protein for haem, 1.66–2.17 nmol/mg protein for protoporphyrin and 0.25–0.40 nmol/mg protein for biliverdin. Possible implications of this previously unreported finding are discussed but they are not considered incompatible with the conclusion that all eggshell pigments are endogenously synthesized in the oviduct system. Copyright © 2011 John Wiley & Sons, Ltd. |
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| Keywords: | haem protoporphyrin biliverdin HPLC/ESI‐MS/MS shell gland quail |
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