Fluorescence optical sensors for continuous determination of near-neutral pH values |
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| Institution: | 1. Department of Pharmacology, Medical University of Silesia, Medyków 18, PL 40-752 Katowice, Poland;2. Department of Internal Medicine and Clinical Pharmacology, Medical University of Silesia, Medyków 18, PL 40-752 Katowice, Poland;3. Department of Biophysics, School of Pharmacy and Laboratory Medicine, Medical University of Silesia, Jedności 8, PL 41-200 Sosnowiec, Poland;4. Department of General and Vascular Surgery, Medical University of Silesia, Ziołowa 45/47, PL 40-635 Katowice, Poland |
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| Abstract: | The preparation and performance of two types of optical sensors for continous measurement of near-neutral pH values are described. the sensors are based on glass-immobilized fluorescent pH indicators and allow the determination of pHs in the range 6.4 to 7.7 with a precision of ±0.01 units. Response times are of the order of 1 min for 99% of the total signal change. Adverse effects of ionic strength are almost completely eliminated by appropriate treatment of the glass surface, thereby creating a well-defined and highly charged environment for the indicator. When the sensing layers are attached to the end of a bifurcated fibre optical light guide, a device for remotely sensing pH values is obtained. |
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