| 水溶性羟基化单壁碳纳米管与人血清白蛋白之间的相互作用研究 |
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| 引用本文： | 刘淑芳,尹俊发,宋茂勇,汪海林. 水溶性羟基化单壁碳纳米管与人血清白蛋白之间的相互作用研究[J]. 高等学校化学学报, 2009, 30(9): 1733-1738 |
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| 作者姓名： | 刘淑芳 尹俊发 宋茂勇 汪海林 |
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| 作者单位： | 中国科学院生态环境研究中心, 环境化学与生态毒理学国家重点实验室, 北京 100085 |
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| 基金项目： | 中国科学院知识创新工程青年人才领域前沿项目(批准号:RCEES-QN-200715)资助 |
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| 摘 要： | 利用荧光光谱、UV吸收光谱、同步荧光光谱和透射电子显微镜等技术较为系统地研究了水溶性羟基化单壁碳纳米管与人血清白蛋白(HSA)的相互作用. 实验发现, 这种羟基化单壁碳纳米管可以明显猝灭HSA的内源荧光, 且猝灭效应随碳纳米管浓度增大而增强. 同时, HSA对水溶性羟基化单壁碳纳米管起到一定的分散和稳定作用. 同步荧光光谱表明, 二者之间的相互作用可导致HSA的构象发生变化, HSA的色氨酸残基荧光信号发生2 nm的红移, 表明色氨酸残基周围微环境的极性降低.
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| 关 键 词： | 碳纳米管 人血清白蛋白 透射电子显微镜 荧光 同步荧光 荧光猝灭, |
| 收稿时间： | 2008-09-01 |
Interaction Between Water-soluble Hydroxylated Single-wall Carbon Nanotubes and Human Serum Albumin |
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| LIU Shu-Fang,YIN Jun-Fa,SONG Mao-Yong,WANG Hai-Lin. Interaction Between Water-soluble Hydroxylated Single-wall Carbon Nanotubes and Human Serum Albumin[J]. Chemical Research In Chinese Universities, 2009, 30(9): 1733-1738 |
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| Authors: | LIU Shu-Fang YIN Jun-Fa SONG Mao-Yong WANG Hai-Lin |
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| Affiliation: | State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 10085, China |
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| Abstract: | The interaction between water-soluble hydroxylated single-wall carbon nanotube and human serum albumin and other related effects were investigated using fluorescence spectroscopy,absorption spectroscopy,synchronous fluorescence spectroscopy and transmission electron microscopy(TEM)methods.Fluorescence quenching of human serum albumin by a hydroxylated single-wall carbon nanotube was observed,indicating the interaction between them occurs.TEM images show the hydroxylated carbon nanotubes system can be stabil... |
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| Keywords: | Carbon nanotubes Human serum albumin Transmission electron microscopy(TEM) Fluorescence Synchronous fluorescence Fluorescence quenching |
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