新型钙荧光探针在H2O2对HL-60胞浆钙影响研究中的应用

采用H2O2、黄嘌呤和黄嘌呤氧化酶、H2O2/抗坏血酸/Fe^2＋（或H2O2/Fe^＋）、H2O2和辣根过氧化酶（HRP）等不同活性氧产生体系,对自行合成的胞浆特异性钙荧光探针STDIn的活性氧耐受性进行了考察.以STDIn-AM为探针标记HL-60细胞,激光共聚焦技术检测单细胞内Ca^2＋-STDIn荧光强度的变化;探讨了不同浓度的H2O2对胞内Ca^2＋浓度变化的影响,从膜结构和生物信号传导的角度研究了氧自由基对培养细胞的生物效应.

Studies on the Effect of Reactive Oxygen on the Intracellular Ca2+ Levels of HL-60 Cells with a New Fluorescence Ca2+ Probe

The effects of reactive oxygen species on the spectral properties of the new fluorescence Ca~(2+) probe STDIn were evaluated using the different oxidant-generating systems.The effect of reactive oxygen on the intracellular Ca~(2+) levels of HL-60 cells labeled with the fluorescence Ca~(2+) probe STDIn was studied by laser scanning confocal microscope.The results were shown that the new fluorescence Ca~(2+) probe STDIn exhibits great advantage flou-3 and fura-2,and the intracellular Ca~(2+) levels of HL-60 cells were related to the dosage of hydrogen peroxide.