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Biofuel cell anode based on the Gluconobacter oxydans bacteria cells and 2,6-dichlorophenolindophenol as an electron transport mediator
Authors:S. V. Alferov  L. G. Tomashevskaya  O. N. Ponamoreva  V. A. Bogdanovskaya  A. N. Reshetilov
Affiliation:(1) Pushchino State University, Pushchino, Russia;(2) Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow oblast, 142290, Russia;(3) Tula State University, pr. Lenina 92, Tula, 300600, Russia;(4) Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences, Leninskii pr. 31, Moscow, 119071, Russia
Abstract:A basic scheme of the use of the Gluconobacter oxydans bacteria cells as a biocatalyst at an anode of a biofuel cell with air-based cathode is raised up. The anode and cathode of the cell are made of graphite; 2,6-dichlorophenolindophenol serves as an electron transport mediator; and glucose is the substrate to be oxidized. The open-circuit voltage is 55 mV, for the bacteria cell, the mediator, and glucose concentrations of 3 mg/ml (raw weight), 34 mM, and 10 mM, respectively. The voltage and current of the biofuel cell loaded with an external resistance of 10 kohm are 5.6 mV and 0.56 mA. The cell’s internal resistance is 88 kohm.
Keywords:biofuel cell  bacteria cells  anode  mediator  glucose
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