Enzyme-linked immunosorbent assay for detection of organophosphorylated butyrylcholinesterase: a biomarker of exposure to organophosphate agents |
| |
Authors: | Wang Limin Du Dan Lu Donglai Lin Chiann-Tso Smith Jordan N Timchalk Charles Liu Fengquan Wang Jun Lin Yuehe |
| |
Institution: | Laboratory of Analytical Chemistry and Centre for Process Analytical Chemistry and Sensor Technology ProSens, ?bo Akademi University, ?bo, Finland. |
| |
Abstract: | Lowering of the detection limit of solid-state lead-selective electrodes was achieved by using the tuned galvanostatic polarization method. A Nernstian response was obtained down to nanomolar concentrations (low detection limit 10(-9) mol dm(-3)Pb(2+)). Good repeatability of the calibration curves was achieved by using a well established measuring procedure. Relatively high cathodic current densities were applied to the solid-state membrane in order to shorten the measurement time. Successful determination of lead in a synthetic sample (pPb(2+)=7.97±0.08) was achieved by introducing an analytical protocol and favourably compared to inductively coupled plasma mass spectrometry (pPb=7.93). By applying this method, a significant improvement in the detection limit of solid-state lead-selective electrodes was attained. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |