Quantitative analysis of thymine with surface-enhanced Raman spectroscopy and partial least squares (PLS) regression |
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| Authors: | Lei Zhang Qingqing Li Wei Tao Bohao Yu Yiping Du |
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| Institution: | 1. Key Laboratory for Advanced Materials and Research Center of Analysis Test, East China University of Science and Technology, 200237, Shanghai, China
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| Abstract: | Silver sol surface-enhanced Raman spectroscopy (SERS) was considered as a technique in the quantitative analysis of low-concentration thymine. Because of the poor stability and reproducibility of SERS signal, a polymer of polyacrylic acid sodium was selected as a stable medium to add into silver sol in order to obtain a surface-enhanced Raman spectroscopy signal. Assignments of Raman shift for solid thymine, SERS of thymine, and SERS of thymine containing stable medium were given. The comparison of Raman peaks between them showed that the addition of stable medium had a little influence on the SERS of thymine and is suitable for the quantitative analysis of low-concentration thymine. |
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