| 悬浮培养红豆杉细胞团区域化与营养生理的关系 |
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| 引用本文： | 李春,元英进,等.悬浮培养红豆杉细胞团区域化与营养生理的关系[J].天津大学学报(自然科学与工程技术版),2001,34(1):51-53. |
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| 作者姓名： | 李春 元英进 |
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| 作者单位： | 李春（天津大学化工学院，天津 300072）
元英进（天津大学化工学院，天津 300072）
胡宗定（天津大学化工学院，天津 300072） |
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| 基金项目： | 教育部"跨世纪优秀人培养计划"基金和天津市重点攻关项目资助(98311811) |
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| 摘 要： | 通过对悬浮培养的南方红豆杉（Ｔaxus chinensis var.Mairei)细胞团切片及胞内淀粉颗粒染色等形态学上的系统观察，发现细胞团从里向外呈现不同层次，淀粉颗粒主要分布在细胞外层。对胞内淀粉、可溶性蛋白、细胞生物量和紫杉醇产量进行动态测定，表明细胞干重与胞内淀 粉和蛋白量呈正相关，从对淀粉代谢情况看，可以初步认为细胞团不同层次细胞周围营养物质的浓度梯度是细胞形态与功能分化的重要原因。
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| 关 键 词： | 细胞团区域化 营养生理 紫杉醇 细胞培养 南方红豆杉 |
| 文章编号： | 0493-2137（2001）01-0051-03 |
| 修稿时间： | 2000年2月28日 |
STUDIES ON NUTRITION
PHYSIOLOGY AND COMPARTMENTALIZATION OF CELL AGGREGATES IN CELL SUSPENSION CULTURE OF TAXUS
CHINENSIS VAR.MAIREI |
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| LI Chun,YUAN Ying-jin,HU Zong-ding.STUDIES ON NUTRITION
PHYSIOLOGY AND COMPARTMENTALIZATION OF CELL AGGREGATES IN CELL SUSPENSION CULTURE OF TAXUS
CHINENSIS VAR.MAIREI[J].Journal of Tianjin University(Science and Technology),2001,34(1):51-53. |
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| Authors: | LI Chun YUAN Ying-jin HU Zong-ding |
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| Abstract: | By observing the section of cell aggregates under an optical microscope and measuring the content of starch grains,soluble protein,biomass and taxol,it was found that there were different compartments in cell aggregates.The starch grains were distributed mainly in the surfacce layer.In the case of starch metabolism,it was preliminarily demonstrated that these compartments have different functions,and it was proposed that the nutrient gradient was an important reason of differentiation of morphology and function of cell compartments in cell aggregates in cell suspension culture. |
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| Keywords: | compartmentalization nutrition physiology taxol plant cells culture taxus chinensis var mairei |
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