基于纳米CeO_2增敏鲁米诺化学发光法测定对乙酰氨基酚的研究

基于碱性条件下,CeO_2纳米粒子能够有效增敏鲁米诺-KMnO_4体系的化学发光,并结合流动注射技术建立了一种对乙酰氨基酚测定的新方法。实验研究了影响化学发光检测信号的多种因素,并初步探讨了可能的化学发光机理。在最佳实验条件下,对乙酰氨基酚浓度在1.0×10-7~5.0×10-5mol/L范围内与相对化学发光强度的抑制值呈良好的线性相关,相关系数(r2)为0.996 4,检出限(3σ)为3.3×10-8mol/L。对5.0×10-6mol/L的对乙酰氨基酚溶液平行测定11次,计算得相对标准偏差(RSD)为0.3%。该法用于银翘片中对乙酰氨基酚含量的测定,回收率为98.0%;对尿液的加标回收率为97.9%~98.7%,结果满意。

Study on Determination of Acetaminophen by CeO2 Nanoparticle-sensitized Luminol Chemiluminescence

A new method for the determination of acetaminophen was established by flow injection technique as CeO2 nanoparticles(CeO2 NPS) could effectively enhance the chemiluminescent(CL) intensity of luminol-KMnO4 system in alkaline conditions.In this study,a variety of factors influencing the chemiluminescence detection signal were studied,while the possible CL mechanism was discussed.Under the optimum experimental conditions,the concentration of acetaminophen was linear with the inhibition of relative CL intensity in the range of 1.0 × 10-7 mol/L-5.0 × 10-5 mol/L.The detection limit(3σ) was 3.3 × 10-8 mol/L.And the relative standard deviations(RSD) were calculated to be 0.3％ for 11 times parallel detection on 5.0 × 10-6mol/L acetaminophen solution.The method was successfully applied in the determination of acetaminophen in Yinqiao tablets and urine with the satisfactory recoveries of 98.0％ and 97.9％-98.7％,respectively.