Chromium localization in plant tissues of Lycopersicum esculentum Mill using ICP-MS and ion microscopy (SIMS) |
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Authors: | Pedro Antonio Mangabeira Konstantin L Gavrilov Arno Heeren Oliveira Maria Isabel Severo Delmira da Costa Silva Françoise Escaig Marcelo Schramm Mielke Pierre Galle |
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Institution: | a Service Central d’Analyse (SCA), Chemin du Canal, Echangeur de Solaize BP-22-69390, Vernaison, France b Department of Biology, University Santa Cruz, Km 22 Rodovia Ilhéus-Itabuna, CEP 45650-000 Ilhéus, Bahia, Brazil c Enrico Fermi Institute, The University of Chicago, IL, USA d Service de Physique-Chimie, Ecole Nationale Vétérinaire d’Alfort, Maison Alfort, France |
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Abstract: | High-resolution imaging secondary ion mass spectrometry (HRI-SIMS) in combination with inductively coupled plasma mass spectrometry (ICP-MS) were utilised to determine specific sites of chromium concentration in tomato plant tissues (roots, stems and leaves). The tissues were obtained from plants grown for 2 months in hydroponic conditions with Cr added in a form chromium salt (CrCl3·6H2O) to concentrations of 25 and 50 mg/L. The chemical fixation procedure used permit to localize only insoluble or strongly bound Cr components in tomato plant tissue. In this work no quantitative SIMS analysis was made. HRI-SIMS analysis revealed that the transport of chromium is restricted to the vascular system of roots, stems and leaves. No Cr was detected in epidermis, palisade parenchyma and spongy parenchyma cells of the leaves. The SIMS-300 spectra obtained from the tissues confirm the HRI-SIMS observations. The roots, and especially walls of xylem vessels, were determined as the principal site of chromium accumulation in tomato plants. |
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Keywords: | Ionic microscopy Microanalyses Heavy metal |
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