| A Study on Anti-oxidative Activity of Soybean Peptides with Linoleic Acid Peroxidation Systems |
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| 引用本文： | XU Li LI Hong-mei HUANG Yi-bing WANG Hua NIE Guang-jun ZHANG Xue-zhong. A Study on Anti-oxidative Activity of Soybean Peptides with Linoleic Acid Peroxidation Systems[J]. 高等学校化学研究, 2006, 22(2): 205-208. DOI: 10.1016/S1005-9040(06)60077-9 |
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| 作者姓名： | XU Li LI Hong-mei HUANG Yi-bing WANG Hua NIE Guang-jun ZHANG Xue-zhong |
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| 作者单位： | XU Li 1，LI Hong-mei 2，HUANG Yi-bing 1，WANG Hua 3，NIE Guang-jun 1 and ZHANG Xue-zhong 1**1. Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education，Jilin University，Changchun 130021，P. R. China;2. College of Food Engineering，Jilin Agricultural University，Changchun 130018，P. R. China;3. China-Japan Union Hospital of Jilin University，Changchun 130033，P. R. China |
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| 基金项目： | Supported by the Grant from ＂The Fifteenth＂ Science and Technology Plan of China（ No. 2001BA747C）. |
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| 摘 要： | Introduction Freeradicalsproducedbyperoxidationcancause cellulardamageandforfeitureofavarietyofphysiologi calfunctions[1,2].Inrecentyears,ithasbeenreported thatsomenon enzymicsmallmolecularcompounds,suchasVitaminC(VC)andVitaminE(VE),glutathi one(GSH),SODm…
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| 关 键 词： | 大豆生物活性缩氨酸 抗氧化活性 亚油酸过氧化反应 蛋白质 成分分析 |
| 文章编号： | 1005-9040（2006）-02-205-04 |
| 收稿时间： | 2005-12-20 |
A Study on Anti-oxidative Activity of Soybean Peptides with Linoleic Acid Peroxidation Systems |
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| Li XU, Hong-mei LI, Yi-bing HUANG, Hua WANG, Guang-jun NIE,Xue-zhong ZHANG,. A Study on Anti-oxidative Activity of Soybean Peptides with Linoleic Acid Peroxidation Systems[J]. Chemical Research in Chinese University, 2006, 22(2): 205-208. DOI: 10.1016/S1005-9040(06)60077-9 |
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| Authors: | Li XU Hong-mei LI Yi-bing HUANG Hua WANG Guang-jun NIE Xue-zhong ZHANG |
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| Affiliation: | aKey Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, Jilin University, Changchun 130021, P. R. China;bCollege of Food Engineering, Jilin Agricultural University, Changchun 130018, P. R. China;cChina-Japan Union Hospital of Jilin University, Changchun 130033, P. R. China |
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| Abstract: | Soybean bioactive peptides(SBPs) were prepared from the isolated soybean protein by proteolysis with an alkaline protease, alcalase, at 50 ℃ and pH = 8. 0. The dependence of hydrolysis time on hydrolysis degree and molecular weight distribution were examined. The hydrolysate was fractionated on a Sephadex G-25 column and the anti-oxidative activities of the fractions were detected by the method of pyrogallol auto-oxidation. The average chain length of soybean peptides that have anti-oxidative activity was estimated to be about 7. The anti-oxidative properties of the soybean peptide were also studied by using linoleic acid peroxidation systems. The optimal condition of the peroxidation system was set up, Vc/Cu2 as the inducer at pH = 7.4 and 25 ℃. In addition, soybean peptides show higher antioxidative activity compared with GSH. |
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| Keywords: | Soybean bioactive peptides Anti-oxidative activity Linoleic acid peroxidation system |
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